Midori Green Advance

Midori Green Advance was developed to offer a non carcinogenic and less mutagenic dye for agarose electrophoresis.

Besides this positve effects for your health this dye shows a very high sensitivity even for small fragments as well as a superior signal to noise ratio.

Even for staining of RNA molecules the dye can be used very well. There is an application note available (see Documents).

Midori Green Advance can be used for regular UV tables as well as for new LED based instruments

First comparison

Ethidium Bromide & UV light

MG Advance & UV light

MG Advance & LED illuminator

Second comparison

Fig 2: Comparison of sensitivity between Ethidium Bromide (left) and Midori Green Advance (right) using an UV-Transilluminator (invers projection). 
Data kindly provided by Ms. Abel, Heinrich-Heine-University Düsseldorf; Germany.

Customer Feedback

"...we have tested Midori Green Advance and we are very excited about it. Please find attached to this email a typical picture...."

Dr. Olaf Voolstra,
Biosensor Research Group, Hohenheim University
Stuttgart

Precasting

1. Prepare 100 ml of agarose gel solution (concentration from 0.8-3.0%) and heat until the solution is completely clear and no small floating particles are visible.
2. Add 4-6 µl of Midori Green Advanced DNA Stain to the gel solution and mix it gently. Cool the gel to 50- 60ºC and cast the gel, into the gel tray. When the gel is solid, load the samples and perform electrophoresis.
3. Detect the bands using a UV or LED illuminator. Yellow or green gelatin- or cellophane filters should be used for photography.

Poststaining

1. Midori Green Advanced DNA Stain poststaining solution may be used 2-3 times.
2. Staining solution that is going to be reused should be preferably stored at room temperature in the dark.
3. For <0.5 cm thick agarose gel, 10-25 µl of the stain should be used per 100 ml of buffer.
4. Optimal staining time (5 - 60 minutes) and the amount of the stain may depend on the thickness of the gel

NOTICE: Usage is not recommended with SDS containing loading buffers because of band appearance caused by stain and SDS interaction!