High yield - High purity - Fast preparation
The gel demonstrates that FastGene® Plasmid Mini Kits yield equal amount of plasmid DNA compared to other supplier with even more time consuming protocols. The preparation with the FastGene® Plasmid Mini Kit was performed by using the Fast Protocol.
Fig. 1: pBluescript plasmid DNA was isolated from a 1,4 ml E.coli culture (DH5α in LB medium) according to the recommended
procedures of different kits and eluted in 50 µl elution buffer. 2 µl of each eluate were loaded on a 0,7% TAE agarose gel.
Fig.2: Typical fluorescent capillary sequencing result from pBluescript plasmid DNA purified using the FastGene® Plasmid Mini Kit. Cycle sequencing reaction was performed on an ABI3100 sequenzer. 1 µl of purified plasmid DNA was used as a template. Dye terminators were removed by FastGene® Dye Terminator Removal Kit
