Direct PCR

From cells to PCR in 15 minutes
Long time storage at -20 °C
Saliva
Hair roots
Animal tissue
Mouse tails
Plants
- Leaf
- Blossom
- Pollem
Yeast

Details

Go from cells to PCR in less than 15 mins!

Nippon Genetics is proud to introduce a novel reagent specially formulated in order to eliminate nucleic acid purification for PCR analysis. DNAreleasy Advance is a continuous development of our well known lysis reagent DNAreleasy suitable for template preparation of PCR experiments. DNAreleasy Advance replaces time consuming and tedious extraction and purification methods. The released DNA can be used directly in a PCR reaction or can be stored at -20 °C for several months.

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Applications

Genomic DNA from scallops

Genomic DNA scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The agarose gel shows the high yield of amplified DNA fragment.

Direct PCR

The same amplified DNA was used for subsequent sequencing experiments.

Sequencing after direct PCR

Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany.

Quantitative PCR of plant tissues

Total DNA was isolated by DNAreleasy Advance from various plant species. Thereafter the melting curve was determined by RT PCR by using a Light Cycler instrument (Roche):

Positive control (PTC), maize meal, wheat flour, sugar beet, white cabbage, negative control (NTC)

Human and animal genomic DNA

Genomic DNA was isolated by using DNAreleasy Advance from different materials and organisms and analyzed by Roche's Light Cycler (Cytb primer):

human tissue direct PCR

(1) Positive control human DNA, (2) saliva, (3) hair root, (4) pig liver, (5) drosophila melanogaster, (6) horse meat

DNA Releasy

DNAreleasy

DNAreleasy Advance replaces time consuming and tedious extraction and purification methods. The released DNA can be used directly in a PCR reaction or can be stored at -20 °C for several months.

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