Direct PCR kit

The Fastgene^® Direct PCR Kit is the optimal combination of our new developed lysis reagent DNAreleasy Advance and an engineered enzyme from Kapa Biosystems.

The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for consolidation of PCR cycling protocols and reaction conditions while increasing success rates. The high performance of the KAPA2G Robust DNA Polymerase eliminates the need for multiple enzymes and protocols - standardize your PCRs with a single enzyme solution.

 The Fastgene Direct PCR Kit offers:

• Ultrafast lysis procedure (15 minutes, no hands on time) of genomic DNA from a lot of different sample material.
• DNA can be used directly in PCR experiments or stored at -20°C for several months.
• Hot Start technology using antibody mediated PCR enzyme
• User freindly Ready Mix format minimize pipetting errors
• Robust performance across a wide range of GC- and AT-rich templates becaseu of the Kapa 2G Robust HS Ready Mix
• Increased PCR success rates
• Consolidated PCR protocols and reaction conditions with a single enzyme
• Improved tolerance to common PCR inhibitors for crude samples and/or DNA extractions
• Three novel buffers and a proprietary PCR enhancer offer extended optimization options for the most difficult templates
• Time and cost saving procedure

Genomic DNA from scallops

Genomic DNA scallops was isolated by DNAreleasy Advance and a part of the supernatant was directly added to the PCR reaction. The PCR reaction was perfomed by using Kapa Biosystems 2GRobust Hot Start Ready Mix. The agarose gel shows the high yield of amplified DNA fragment.

The same amplified DNA was used for subsequent sequencing experiments.

Data kindly provided by Dr. Schubbert, Eurofins Medigenomix GmbH, Ebersberg, Germany.

Genomic DNA from various plant species

Total DNA was isolated by DNAreleasy Advance from various plant species and amplified with KAPA 2G Robust.

(1) Marker, (2) positive control, (3) negative control, (4) white cabbage (leaf), (5) sugar beet (leaf), (6) maize meal, (7) soja, (8) canola, (9) wheat flour.