Optima(l) robustness for very complex samples
The FastGene® Optima can handle very complicated templates. The highly purified Taq polymerase gives high efficiency while the proof-reading polymerase guarantees the fidelity. The robustness of both enzymes makes the amplification of complex tissue, such as liver (Fig. 1), possible.
Fig.1: Comparison between (A) Competitor T‘s and (B) FastGene® Optima polymerase mixture using the hard to amplify catshark liver DNA as template. The PCR product has a size of 1030 bp and was separated onto an 1.2% agarose gel. The FastGene® Optima produces much less primer dimers and has a higher amplification efficiency.
Optima(l) efficiency for GC-rich templates
Most polymerases have a very low amplification efficiency if the template DNA is GC-rich. As seen in Fig. 2, the FastGene® Optima has an excellent amplification efficiency even with GC-rich templates, which is even higher compared to the efficiency of polymerases especially designed for GC-rich templates (Fig. 2).
Fig. 2: Comparing the ability of Competitor T‘s and FastGene® Optima polymerase mixture to amplify GC-Rich DNA fragments. Two fragments of 60.7 % and 64.3% were amplified resulting in two products of 1839 bp and 1260 bp, repectively. FastGene® Optima had a higher efficiency compared to Competitor T‘s polymerase mixture.
Data was kindly provided by Ms. Ryoko Nakayama, Department of Pathology, Tsurumi University, Japan.
Optima(l) for SNP-typing
The detection of single nucleotide polymorphism (SNP) requires extreme fidelity. The proof-reading activity guarantees this needed fidelity (see Fig.3).
Fig. 3: SNP typing of the ALDH gene using FastGene® Optima polymerase. The ALDH classified as human sensitivity to alcohol gene was analysed for presence of a SNP by digesting the amplification of homo- and heterozygotes using MboII.
Data was kindly provided by Dr. Che Xiano-Fang, Department of Biochemistry, Tokyo Medical University, Japan.
