Taq HotStart

Fig. 1: Reactions (25 µl) were set up according to manufacturer’s instructions, with 20 ng of hgDNA as template, and 0.5 µM of each primer. DMSO was included at a final concentration of 5%. PCR was performed for a total of 35 cycles.

Fig. 2: Reactions (25 µl) were set up according to manufacturer’s instructions, with 25 ng of hgDNA as template,
and 0.5 µM of each primer. Qiagen Q-solution was included at a final concentration of 1X. PCR was performed for a total of 35 cycles.