IC Green qPCR

No inhibition

It is well-known that SYBR green is extensively inhibiting the qPCR. This fact led to the development of SYBR resistant enzymes. An alternative approach is to develop a dye that does not inhibit the reaction. This dye is named FastGene® IC Green. FastGene® IC Green is an intercalating dye, only detecting double stranded DNA. By not inhibiting the reaction, the FastGene® IC Green enhances the processitivity enabling the detection of genes at a lower CT-value. The superior buffer chemistry enables the detection of low copy number genes, which with other dyes could not be detected. The comparison to competitors shows that FastGene^® IC Green is one of the best qPCR mixes available. This has been confirmed by customers analyzing many different genes.

Robust chemistry for faster results

The FastGene® IC Green buffers were designed to have a superior robustness. This guarantees the linearity of the qPCR and creates a better accuracy, essential for reproducible results. Additionally, qPCRs can be performed at lower amplifcation times for example using fast protocols.

Universal and consistant - get reproducible results at any qPCR instrument

The FastGene® IC Green kit is universal. The reference dyes come in a separate vial and can be added once to the master mixes. Hence, this kit can be used with qPCR instruments which need a high ROX™ concentration as well as instruments that need a low concentration or no ROX™. There is even a special version with fluorescein.