The PiNK Prestained Protein Ladder contains 11 proteins (8 pink and 3 blue bands) that resolve into sharp, tight bands in the range of 5-175 kDa. The PiNK Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Quality Control
Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.
Storage Buffer
20 mM Tris-phosphate pH 7.5 (at 25°C ), 2 % SDS, 1 mM
Dithiothreitol, 4.8 M Urea and 12 % (v/v) Glycerol.
(Approximately 0.2 ~ 0.4 mg/ml of each protein in the buffer)
Storage
Store at -20 °C for 12 months.
Stable at +4 °C for 3 months.
Recommendations for Loading
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
- 5 μl per well for mini-gels, 2.5 μl per well for blots
- 10 μl per well for large gels, 5 μl per well for blots
