The FastGene^® triple coloured prestained protein ladders have prestained proteins covering a wide range molecular weights:

BlueStar	BlueStar PLUS	BlueEasy

These Prestained Protein Ladders are designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins. 

The ladders aresupplied in gel loading buffer and is ready to use. Do not heat, dilute, add reducing agent before loading.

Quality Control

Tested in SDS-polyacrylamide gel electrophoresis and Western blotting. 

Storage Buffer

20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol.

Storage

Store at -20°C for 12 months.
Stable at +4°C for 3 months.

Recommendations for Loading

1.Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2.Mix thoroughly, to ensure that the solution is homogeneous.
3.Load the following volumes of the ladder on SDS-polyacrylamide gel:
   - 5 μl per well for mini-gels, 3 μl per well for blots;
   - 10 μl per well for large gels, 6 μl per well for blots

• Monitoring of protein migration during SDS-polyacrylamide gel electrophoresis.

• Monitoring of protein transfer onto membranes during Western blotting.

• Sizing of proteins on SDS-polyacrylamide gels and Western blots.

BlueStar Prestained Protein Marker (Cat. No. MWP03 Size: 500 μl)

• Broad range:9 - 180 kDa.
• High performance: 12 bands with 3 colours (blue, red and green)
• Ready to use: Supplied in a loading buffer for direct loading on gels.
• Easy to identify: The 28 (green) and 75 kDa (red) are reference bands.
• Sharp bands

Applications

The BlueStar PLUS Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Quality Control

Under suggested conditions, BlueStar Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

Storage

Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Loading

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

All products are for research use only and not intended for human or animal diagnostic or therapeutic uses

BlueStar PLUS Prestained Protein Marker (Cat. No. MWP04 Size: 500 μl)

• Broad range:9 - 245 kDa.
• High performance: 12 bands with 3 colours (blue, red and green)
• Ready to use: Supplied in a loading buffer for direct loading on gels.
• Easy to identify: The 25 (green) and 75 kDa (red) are reference bands.
• Sharp bands

Applications

The BlueStar PLUS Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

Quality Control

Under suggested conditions, BlueStar PLUS Prestained Protein Ladder resolves 13 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose membrane.

Storage

Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Loading

3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.

All products are for research use only and not intended for human or animal diagnostic or therapeutic uses

BlueEasy Prestained Protein Marker (Cat. No. MWP06 Size: 500 μl)
 

• Broad range:6.5 - 270 kDa.
• High performance: 10 bands with 3 colours (blue, orange and green)
• Ready to use: Supplied in a loading buffer for direct loading on gels.
• Easy to identify: The 52 (green), 30 kDa and 270 kDa (orange) are reference bands.
• Sharp bands

Applications

The BlueEasy Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (62,5 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 10 mM Dithiothreitol, 1 mM EDTA, and 33 % (v/v) Glycerol).
 

Quality Control
Under suggested conditions, BlueEasy Prestained Protein Ladder resolves 10 major bands in polyacrylamide gel with appropriate buffers and after Western blotting to nitrocellulose and other membranes.

Storage
Stable for up to 2 weeks at 25°C. Stable for up to 3 months at 4°C. For long term storage, store at -20°C.

Loading
3 μl or 5 μl per loading for clear visualization during electrophoresis on 15-well or 10-well mini-gel, respectively.

1.5~2.5 μl per well for general Western transferring. Apply more for thicker (> 1.5 mm) or larger gel.